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RE: amino

in #science7 years ago

Oh, absolutely. It's fairly common since it's so gentle and non-toxic.

The biggest problem is that it's also slow. We usually add resazuring as a redox indicator at the same time, prior to inoculation. We have to wait for it to go from pink to clear, and this can take a few hours at 37 C, even if we prepare using water boiled under N2/CO2 and keep an anoxic headspace and it's just scavenging the last traces of oxygen.

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Forgot to add, we generally schedule anaerobic media prep around dinner/pub time for that reason.