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Just use one loose knot. The WAXED dental floss then does all the work to prevent slippage. This guy let himself go within a minute of the filming. I once had someone screaming at me for being so cruel. I replied, "Really, because a lot of people will just kill them. Let's see if he cares." I untied the bee and let it crawl around on my hand for a while before it flew away. They were amazed that it didn't sting me. I told them that "Perhaps he wasn't as angry about it" as they were. I've done it probably almost 100 times, and the bees always fly away just fine.